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Serous fluid1/26/2024 Monoclonal antibody fluorescence for routine lymphocyte subpopulation analysis with the Abbott CELL-DYN Sapphire haematology analyser. Molero T, Lemes A, De la Iglesia S, Scott CS. Pericardial fluid is the serous fluid secreted by the serous layer of the pericardium and is typically echo free by ultrasound. European multi-center evaluation of the Abbott Cell-Dyn Sapphire hematology analyzer. Müller R, Mellors I, Johannessen B, Aarsand AK, Kiefer P, Hardy J, et al. 10.1309/AJCPY7J7OLASZAPC Search in Google Scholarĥ. Use of the Cell-Dyn Sapphire hematology analyzer for automated counting of blood cells in body fluids. De Smet D, Van Moer G, Martens GA, Nanos N, Smet L, Jochmans K, et al. 10.1309/AJCPOUSCFIW60DYC Search in Google ScholarĤ. Hematology analyzers and body fluid analysis. Modifications of haematology analyzers to improve cell counting and leukocyte differentiating in cerebrospinal fluid controls of the Joint German Society for Clinical Chemistry and Laboratory Medicine. Kleine TO, Nebe CT, Löwer C, Lehmitz R, Kruse R, Geilenkeuser W-J, et al. ![]() Diagnosis, treatment and prophylaxis of spontaneous bacterial peritonitis: a consensus document. Rimola A, Garcia-Tsao G, Navasa M, Piddock LJ, Planas R, Bernard B, et al. However, when optimized manual gating strategy is used, a good correlation and negligible bias were found.Ĭonclusions: We have demonstrated that with a modified algorithm, CELL-DYN Sapphire will provide reliable identification and enumeration of blood cells in peritoneal and pleural fluidsġ. felt that the resulting increase in size and expense of First it is intended for use at the bench. Results: The standard Sapphire algorithm showed substantial deviations from the reference microscopic differentiation: polymorphonuclear cell counts were too high because they contained some monocytic cells. It is hoped that this book may serve two purposes. Additionally, we performed optimized manual gating of the Sapphire raw data file using standard flow cytometry software. Methods: A total of 105 serous fluids (39 peritoneal and 66 pleural effusions) were analyzed by the CELL-DYN Sapphire using standard whole-blood algorithm. The aim of the present study was to evaluate the analytical performance of the CELL-DYN Sapphire hematology analyzer for automated differentiation of cells in serous fluids and to explore whether manual analysis of the raw data files could improve the differential count compared with reference microscopy. Currently, microscopic analysis is still considered the “gold standard”. ![]() Background: Correct cell enumeration and differential analysis of body fluids are important in the diagnosis and management of several diseases.
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